敲除纖維連接蛋白對子宮腺肌癥子宮內膜細胞侵襲和遷移的影響

2023年4月，中國北京大學第三醫院生殖醫學中心婦產科女性生育促進國家重點實驗室;北京大學第三醫院全國婦產科臨床研究中心;北京大學輔助生殖教育部重點實驗室;北京市生殖內分泌與輔助生殖技術重點實驗室(北京大學第三醫院) (State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China；National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, 100191, China；Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, 100191,   China；Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology (Peking University Third Hospital), Beijing, 100191,China) Jiangman Gao老師研究團隊在《Heliyon》上發表論文：

“The impact of fibronectin knockout on invasion and migration of endometrial cell in adenomyosis”

“敲除纖維連接蛋白對子宮腺肌癥子宮內膜細胞侵襲和遷移的影響”

Abstract：

The present study aimed to investigate the potential effect of fibronectin (FN) in adenomyosis progression. Small guide RNAs were designed to knock down FN expression in Ishikawa cells. The impact of FN on the proliferation, apoptosis, migration, and invasion of the cells was assessed. Cell proliferation was detected using a Celigo Imaging Cytometer system; apoptosis was quantified by flow cytometry; and cell migration and invasion were investigated via transwell assays. Cell proliferation was markedly suppressed in the FN knockout (KO) group compared with the control group, while apoptosis significantly increased. The levels of cell migration and invasion in the KO group were significantly decreased compared with the control group. Our study revealed that downregulation of FN expression is likely to restrain cell proliferation, migration, and invasion in endometrial cells in adenomyosis.

摘要：

本研究旨在探討纖維連接蛋白(FN)在子宮腺肌病進展中的潛在作用。設計小的引導rna來降低石川細胞中FN的表達。觀察FN對細胞增殖、凋亡、遷移和侵襲的影響。采用Celigo成像細胞儀檢測細胞增殖;流式細胞術定量細胞凋亡;transwell法觀察細胞的遷移和侵襲。與對照組相比，FN基因敲除(KO)組細胞增殖明顯受到抑制，細胞凋亡明顯增加。與對照組相比，KO組細胞遷移和侵襲水平明顯降低。研究表明，下調FN表達可能會抑制子宮腺肌病患者子宮內膜細胞的增殖、遷移和侵襲。

該論文中，人子宮內膜癌細胞（Ishikawa細胞系）的體外培養是使用Ausbian特級胎牛血清完成的。欲了解或購買Ausbian特級胎牛血清可以聯系北京締一生物400-166-8600.