高爾基整體膜蛋白4調控頭頸癌細胞增殖、凋亡和細胞周期

2018年3月，內蒙古醫(yī)科大學附屬醫(yī)院(Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia, People’s Republic of China) Yunfei Bai老師研究團隊在《Biosci Rep》上發(fā)表論文：

“Golgi integral membrane protein 4 manipulates cellular proliferation, apoptosis, and cell cycle in human head and neck cancer”

“高爾基整體膜蛋白4調控頭頸癌細胞增殖、凋亡和細胞周期”

Abstract：

The incidence of global head and neck cancer has increased markedly in the last 10 years, and its prognosis is poor, which seriously endangers people's life and health. At present, there are few studies on its pathogenesis. Golgi integral membrane protein 4 (GOLIM4) is a major member of the Golgi apparatus transporter complex, and its role in tumor is unclear. The present study found that GOLIM4 was the key target protein downstream of stromal interaction molecule 1 (STIM1), which can inhibit the proliferation of head and neck cancer cells FaDu (human pharyngeal squamous carcinoma cell) and Tca-8113 (human tongue squamous carcinoma cell) with knockdown of GOLIM4 by lentivirus. And the decreased expression of GOLIM4 induced cellular apoptosis. Further experiments revealed that FaDu cell cycle progression was changed after GOLIM4 silence, G₁
phase arrest and the number of G₂/M cells decreased significantly. It was also found that the cells in S-phase decreased markedly after GOLIM4 was knocked down compared with the control group by 5-bromo-2'-deoxyuridine (BrdU) incorporation experiment. In conclusion, we found that GOLIM4, as the target gene downstream of STIM1, inhibited the proliferation of head and neck cancer, promoted apoptosis, and regulated cell cycle progression, and GOLIM4 is a novel oncogene in head and neck cancer and might help in developing promising targetted therapies for head and neck cancer patients.

摘要：

近10年來，全球頭頸癌發(fā)病率顯著上升，預后較差，嚴重危害人們的生命健康。目前對其發(fā)病機制的研究較少。高爾基整體膜蛋白4 (GOLIM4)是高爾基體轉運體復合體的主要成員，其在腫瘤中的作用尚不清楚。本研究發(fā)現(xiàn)GOLIM4是基質相互作用分子1 (STIM1)下游的關鍵靶蛋白，可以通過慢病毒敲低GOLIM4抑制頭頸部癌細胞FaDu(人咽鱗癌細胞)和Tca-8113(人舌鱗癌細胞)的增殖。而GOLIM4的表達降低可誘導細胞凋亡。進一步實驗發(fā)現(xiàn)，GOLIM4沉默、G1期阻滯后，FaDu細胞周期進程發(fā)生改變，G2/M細胞數(shù)量明顯減少。5-溴-2′-脫氧尿苷(BrdU)摻入實驗也發(fā)現(xiàn)，與對照組相比，GOLIM4敲除后s期細胞明顯減少。綜上所述，科研人員發(fā)現(xiàn)GOLIM4作為STIM1下游的靶基因，可以抑制頭頸癌的增殖，促進細胞凋亡，調節(jié)細胞周期進程，GOLIM4是頭頸癌的一個新的致癌基因，可能有助于開發(fā)頭頸癌患者的靶向治療方法。

該論文中，FaDu(人咽鱗癌細胞)和Tca-8113(人舌鱗癌細胞)的體外培養(yǎng)是使用Ausbian特級胎牛血清完成的。欲了解或購買Ausbian特級胎牛血清可以聯(lián)系北京締一生物400-166-8600.