新甾體皂苷的提取及其細胞毒性研究

2018年3月，第四軍醫大學藥學院藥物研究所；氟氮化工國家重點實驗室；西安現代化學研究所 (Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, China；State Key Laboratory of Fluorine & Nitrogen Chemicals, Xi’an 710065, China；Xi’an Modern Chemistry Research Institute, Xi’an 710065, China) Haifeng Tang老師研究團隊在《Molecules》上發表論文：

“New Steroidal Saponins from the Rhizomes of Paris vietnamensis and Their Cytotoxicity”

“新甾體皂苷的提取及其細胞毒性研究”

Abstract：

Four new spirostanol saponins, named pavitnosides A-D (1-4), with six known steroidal saponins 5-10 were isolated from the rhizomes of Paris vietnamensis. Their chemical structures were determined based on extensive spectroscopic studies and chemical methods. The aglycones of pavitnoside B and pavitnoside C were not reported in previous work. The cytotoxicity of all saponins was evaluated against human glioblastoma U87MG and U251 cell lines. The new spirostanol saponin 1 displayed weak anti-proliferative activity against U87MG cell line and the known saponins 8 and 9 exhibited significant cytotoxicity against the two tumor cell lines, with IC50 values of 2.16 to 3.14 μM, but did not affect the growth of primary cultures of human astrocytes.

摘要：

從越南梧桐根莖中分離到4個新的螺甾醇類皂苷，命名為pavitnosides A-D(1-4)， 6個已知甾體類皂苷5-10。它們的化學結構是根據廣泛的光譜研究和化學方法確定的。前文未報道過其苷元B和C的苷元。評價了各皂苷對人膠質母細胞瘤U87MG和U251細胞系的細胞毒性。新合成的螺甾醇皂苷1對U87MG細胞系具有較弱的抗增殖活性，而已知的皂苷8和皂苷9對兩種腫瘤細胞系具有明顯的細胞毒性，IC50值為2.16 ~ 3.14 μM，但對人星形膠質細胞原代培養物的生長沒有影響。

該論文中，人膠質瘤細胞系U251和U87MG的體外培養是使用Ausbian特級胎牛血清完成的。欲了解或購買Ausbian特級胎牛血清可以聯系北京締一生物400-166-8600.