一種滅活的新型基因型禽腺病毒4對最近在中國出現的雞心包積液綜合征具有保護作用

2017年8月，中國農業科學院哈爾濱獸醫研究所獸醫生物技術國家重點實驗室；江蘇省重大動物傳染病與人畜共患病預防控制協同創新中心(State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China;   Jiangsu Co Innovation Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou 225009, China) Xiaomei Wang老師研究團隊在《Viruses》上發表論文：

“An Inactivated Novel Genotype Fowl Adenovirus 4 Protects Chickens against the Hydropericardium Syndrome That Recently Emerged in China”

“一種滅活的新型基因型禽腺病毒4對最近在中國出現的雞心包積液綜合征具有保護作用”

Abstract：

Since 2015, China has experienced outbreaks of severe hydropericardium syndrome (HPS), associated with a novel genotype and hypervirulent fowl adenovirus serotype 4 (FAdV-4) infection, with a prevalence in various provinces of the country. This has resulted in huge economic losses in the poultry industry. The novel FAdV-4 showed new genome characters, such as the natural deletion of open reading frame (ORF) 19 and ORF 27 (1966 bp), and high pathogenicity toward chickens. These are coupled with severe hydropericardium, inclusion body hepatitis, and mortality rates ranging from 30% to 90%. Although several inactivated and subunit vaccines against the traditional FAdV-4 have been developed, no commercial vaccine against the emerged disease caused by the novel strain has been available until now. The potential risks of infection with this novel hypervirulent FAdV-4 urgently require an effective vaccine. Thus, an inactivated oil-emulsion FAdV-4 vaccine formulated with the novel genotype virus was developed in this study. The vaccine provided a high level of antibody, preferential T helper 2 (Th2) (interleukin-4 secretion) not Th1 (interferon-γ secretion) response, and full protection against a lethal dose of the novel hypervirulent FAdV-4. Therefore, the novel genotype FAdV-4 vaccine is proposed as an attractive candidate to prevent and reduce the spread of HPS in the poultry industry of China.

摘要：

自2015年以來，中國爆發了嚴重心包積液綜合征(HPS)，與一種新型基因型和高毒力禽腺病毒血清型4 (FAdV-4)感染有關，在全國多個省份流行。這給家禽業造成了巨大的經濟損失。新型FAdV-4具有自然缺失開放閱讀框(ORF) 19和ORF 27 (1966 bp)等新的基因組特征，對雞具有較高的致病性。伴有嚴重心包積液、包涵體肝炎，死亡率從30%到90%不等。雖然已經開發出幾種針對傳統FAdV-4的滅活疫苗和亞單位疫苗，但迄今為止還沒有針對這種新菌株引起的新出現疾病的商業疫苗。這種新型高毒力FAdV-4感染的潛在風險迫切需要一種有效的疫苗。因此，本研究研制了以新基因型病毒配制的FAdV-4滅活油乳劑疫苗。該疫苗提供了高水平的抗體，優先的輔助性T細胞2 (Th2)(白細胞介素-4分泌)而不是Th1(干擾素-γ分泌)反應，并對致命劑量的新型高致病性FAdV-4具有充分的保護作用。因此，新型FAdV-4基因型疫苗被認為是預防和減少HPS在中國家禽業傳播的有吸引力的候選疫苗。

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