RNAi介導的HOXD3敲低抑制人RKO細胞的生長

2016年3月，安徽醫科大學**附屬醫院腫瘤科；安慶醫學院臨床醫學系；安徽醫科大學附屬安慶市醫院病理科(Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022;   Department of Clinical Medicine, Anqing Medical College, Anqing, Anhui 246052;   Department of Pathology, The Affiliated Anqing Municipal Hospital of Anhui Medical University, Anqing, Anhui 246003, P.R. China) FANGJUN CHEN老師研究團隊在《ONCOLOGY REPORTS》上發表論文：

“RNAi-mediated HOXD3 knockdown inhibits growth in human RKO cells”

“RNAi介導的HOXD3敲低抑制人RKO細胞的生長”

Abstract：

Numerous studies have shown that the multifunctional Homeobox-containing (HOX) D3 gene is involved in various physiological and pathological processes. To elucidate the role and mechanism of HOXD3 in colorectal cancer (CRC), we measured its expression in five CRC cell lines. After determining that HOXD3 was highly expressed in the human RKO cancer cell line, we used lentiviral-mediated small interfering RNAs (siRNAs) to knock down HOXD3 expression and assessed proliferation, cell cycle distribution, apoptosis and colony formation using cell proliferation, flow cytometric, and colony formation assays. The expression of HOXD3 was strongly suppressed in the RKO cells infected with the lentiviruse expressing an HOXD3 short hairpin RNA (shRNA). The downregulation of HOXD3 expression in RKO cells significantly decreased proliferation and colony formation, and increased apoptosis in vitro, compared to the cells infected with the mock control (p<0.01). Moreover, specific downregulation of HOXD3 led to the accumulation of cells at the G2 phase of the cell cycle. Our findings revealed that the HOXD3 gene promotes CRC cell growth and plays a pivotal role in the development and survival of malignant human colorectal cancer cells.

摘要：

大量研究表明，多功能同源盒(HOX) D3基因參與多種生理和病理過程。為了闡明HOXD3在結直腸癌(CRC)中的作用和機制，我們檢測了其在5種結直腸癌細胞系中的表達。在確定HOXD3在人類RKO癌細胞系中高表達后，我們使用慢病毒介導的小干擾rna (sirna)來敲低HOXD3的表達，并使用細胞增殖、流式細胞術和集落形成試驗來評估增殖、細胞周期分布、凋亡和集落形成。表達HOXD3短發夾RNA (shRNA)的慢病毒感染RKO細胞后，HOXD3的表達被強烈抑制。與模擬對照組相比，下調HOXD3表達可顯著降低RKO細胞的體外增殖和集落形成，增加細胞凋亡(p<0.01)。此外，HOXD3的特異性下調導致細胞在細胞周期的G2期積聚。我們的研究結果表明，HOXD3基因促進CRC細胞生長，在人類惡性結直腸癌細胞的發展和存活中起關鍵作用。

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