Functional analysis of ESM1 by shRNA mediated knockdown of its expression in papillary thyroid cance

2024年1月，昆明醫科大學**附屬醫院核醫學科、中國電子大學四川省人民醫院核醫學系中國科學技術部（1 Department of Nuclear Medicine, The First Affiliated Hospital of Kunming Medical University, Kunming, P. R. China, 2 Department of Nuclear Medicine, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, P.R. China） Hao Wang研究團隊在《PLOS ONE》上發表論文：

“Functional analysis of ESM1 by shRNA-mediated knockdown of its expression in papillary thyroid cancer cells”

“shRNA介導的抑制甲狀腺乳頭狀癌細胞中ESM1表達的功能分析”

Abstract：

Objective

Endothelial specific molecule-1 (ESM1) is implicated as an oncogene in multiple human cancers. However, the function of ESM1 in papillary thyroid cancer (PTC) is not well understood. The current study aimed to investigate the effect of ESM1 on the growth, migration, and invasion of PTC to provide a novel perspective for PTC treatment.

Methods

The expression levels of ESM1 in PTC tissues form 53 tumor tissue samples and 59 matching adjacent normal tissue samples were detected by immunohistochemical analysis. Knockdown of ESM1 expression in TPC-1 and SW579 cell lines was established to investigate its role in PTC. Moreover, cell proliferation, apoptosis, wound healing, and transwell assays were conducted in vitro to assess cell proliferation, migration and invasion.

Results

The findings revealed that ESM1 expression was significantly higher in PTC tissues than that found in paraneoplastic tissues (P<0.0001). Knockdown of ESM1 expression inhibited the proliferation, migration, and invasion of TPC-1 and SW579 cells in vitro. Compared with the control group, the mRNA and protein levels of ESM1 in PTC cells were significantly reduced following knockdown of its expression (P<0.01). In addition, ESM1-knockdown cells indicated decreased proliferation and decreased migratory and invasive activities (P<0.01, P<0.01, P<0.001, respectively).

Conclusions

ESM1 was identified as a major gene in the occurrence and progression of PTC, which

could increase the proliferation, migration, and invasion of PTC cells. It may be a promising

diagnostic and therapeutic target gene.

摘要：

目的：

內皮特異性分子-1 (ESM1)是多種人類癌癥的致癌基因。然而，ESM1在乳頭狀甲狀腺癌(PTC)中的功能尚不清楚。本研究旨在探討ESM1對PTC生長、遷移和侵襲的影響，為PTC的治療提供新的視角。

方法：

采用免疫組化方法檢測53例腫瘤組織樣本和59例匹配的鄰近正常組織樣本PTC組織中ESM1的表達水平。通過在TPC-1和SW579細胞系中敲低ESM1的表達來研究其在PTC中的作用。此外，通過體外細胞增殖、凋亡、傷口愈合和transwell實驗來評估細胞增殖、遷移和侵襲。

結果：

結果顯示，ESM1在PTC組織中的表達明顯高于在副腫瘤組織中的表達(P<0.0001)。在體外實驗中，敲低ESM1表達可抑制TPC-1和SW579細胞的增殖、遷移和侵襲。與對照組相比，PTC細胞中ESM1表達下調后，其mRNA和蛋白水平均顯著降低(P<0.01)。此外，敲低esm1的細胞增殖能力降低，遷移和侵襲能力降低(P<0.01, P<0.01, P<0.001)。

結論

ESM1是PTC發生發展的重要基因，可以促進PTC細胞的增殖、遷移和侵襲。它可能是一個很有前途的診斷和治療靶基因。

在該研究中，對PC-1, SW579和BCPAP人乳頭狀甲狀腺癌細胞、Nthy-ori3-1甲狀腺濾泡上皮細胞的體外培養，均用到了Ausbian特級胎牛血清。欲了解或購買Ausbian特級胎牛血清可以聯系北京締一生物400-166-8600.