內源性瘦素促進ebss誘導的PFCs自噬

2019年8月，云南省異種移植工程研究中心；云南省異種移植工程研究中心；云南省生物資源保護與利用國家重點實驗室；云南農業大學；云南農業大學；動物科學與技術學院；云南農業大學動物醫學院(Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province, Kunming, People’s Republic of China；Xenotransplantation Engineering Research Center in Yunnan Province, Kunming, People’s Republic of China；State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, People’s Republic of China；College of Animal Science and Technology,Yunnan Agricultural University, Kunming,   People’s Republic of China；College of Veterinary Medicine, Yunnan Agricultural University, Kunming，People’s Republic of China) Hong-Ye Zhao老師研究團隊在《ANIMAL CELLS AND SYSTEMS》上發表論文：

“Endogenous leptin promotes autophagy in EBSS-induced PFCs”

“內源性瘦素促進ebss誘導的PFCs自噬”

Abstract：

Leptin is an important adipokine and plays a vital role in animals. However, the role of leptin in the autophagic response of pig fibroblast cells (PFCs) has not been fully elucidated. In this study, we investigated the relationship between leptin and autophagy as well as underlying molecular basis. We found that PFCs treated with EBSS could secrete leptin, and the leptin concentration in the supernatant of leptin transgenic PFCs was higher than that of WT PFCs. We found an increase in LC3-II protein level and a decrease in p62 protein level in treated leptin transgenic PFCs compared with treated WT PFCs. Meanwhile, we observed an increase of autophagosomes by transmission electron microscopy and an enhancement of the accumulation of LC3 puncta in the cytoplasm of treated leptin transgenic PFCs, and these effects were further augmented by Baf A1 treatment. Furthermore, we detected the expression levels of 7 autophagy signaling pathway genes and 17 autophagy-related (ATG) genes by q-PCR. We found that between the two types of EBSS-treated cells 3 genes expression pattern were significantly different among the 7 autophagy signaling pathway genes and 8 genes expression pattern were significantly differernt among the ATG genes. These results indicated that leptin may promote autophagy and involving the downregulation of FOXO1 and LMNA genes via an unknown pathway which causes the upregulation of the 4 genes and the downregulation of 4 genes.

摘要：

瘦素是一種重要的脂肪因子，在動物體內起著至關重要的作用。然而，瘦素在豬成纖維細胞(PFCs)自噬反應中的作用尚未完全闡明。在這項研究中，研究人員探討了瘦素與自噬的關系及其潛在的分子基礎。研究人員發現經EBSS處理的pfc能夠分泌瘦素，并且在瘦素轉基因pfc的上清液中瘦素濃度高于WT pfc。研究人員發現，與處理過的WT pfc相比，處理過的瘦素轉基因pfc中LC3-II蛋白水平升高，p62蛋白水平降低。同時，研究人員通過透射電鏡觀察到，處理過的瘦素轉基因pfc細胞質中自噬體的增加和LC3點的積累增強，這些作用在Baf A1處理下進一步增強。此外，研究人員通過q-PCR檢測了7個自噬信號通路基因和17個自噬相關(autophagy-related, ATG)基因的表達水平。研究人員發現在ebss處理的兩種細胞中，7個自噬信號通路基因中有3個基因的表達模式存在顯著差異，ATG基因中有8個基因的表達模式存在顯著差異。這些結果表明，瘦素可能通過未知的途徑促進自噬，并參與FOXO1和LMNA基因的下調，導致4個基因的上調和下調。

該論文中，WT和瘦素轉基因PFCs細胞的體外培養是使用Ausbian特級胎牛血清完成的。欲了解或購買Ausbian特級胎牛血清可以聯系北京締一生物400-166-8600.