下調RAD54B表達可抑制肺癌細胞增殖，誘導細胞凋亡

2019年12月，貴州省人民醫院胸外科(Department of Thoracic Surgery, Guizhou Provincial People’s Hospital, Guiyang, Guizhou Province, China) Di Liu老師研究團隊在《Journal of International Medical Research》上發表論文：

“Knockdown of RAD54B expression reduces cell proliferation and induces apoptosis in lung cancer cells”

“下調RAD54B表達可抑制肺癌細胞增殖，誘導細胞凋亡”

Abstract：

Objective: RAD54 homolog B (RAD54B), a member of the SNF2/SWI2 superfamily, is implicated in homologous recombination, and high RAD54B expression predicts the prognostic outcomes of lung adenocarcinoma. However, its role in lung carcinogenesis was unclear so this was determined in the present study.

Methods: We evaluated the gene and protein expression of RAD54B in 15 lung adenocarcinoma tissues and matched adjacent healthy lung tissues by real-time PCR, immunohistochemical staining, and western blotting. A549 lung cancer cells were transduced with lentivirus carrying small hairpin RNA (shRNA) against RAD54B (shRAD54B) or control shRNA (shCtrl), and cell proliferation, viability, apoptosis, and caspase 3/7 activity were evaluated.

Results: RAD54B protein expression was significantly higher in lung adenocarcinoma tissues than in healthy lung tissues. RAD54B gene expression was high in A549 cells but was efficiently knocked down using shRAD54B with an infection efficiency of 80% and a knockdown ratio of 72.2% compared with shCtrl. Suppressing RAD54B expression in A549 cells significantly reduced cell proliferation and caspase 3/7 activity, and significantly increased the apoptotic rate.

Conclusions: RAD54B exerts an oncogenic role in lung cancer cell proliferation.

摘要：

摘要：

目的:SNF2/SWI2超家族成員RAD54同源B (RAD54B)參與同源重組，RAD54B的高表達可預測肺腺癌的預后。然而，其在肺癌發生中的作用尚不清楚，因此本研究確定了這一點。

方法:采用實時熒光定量PCR、免疫組化染色和western blotting檢測RAD54B在15例肺腺癌組織及匹配的鄰近健康肺組織中的基因和蛋白表達。用攜帶小發夾RNA (shRNA)的慢病毒將A549肺癌細胞轉導至RAD54B (shRAD54B)或對照shRNA (shCtrl)，觀察細胞增殖、活力、凋亡和caspase 3/7活性。

結果:RAD54B蛋白在肺腺癌組織中的表達明顯高于正常肺組織。RAD54B基因在A549細胞中表達量較高，但與shCtrl相比，shRAD54B可有效敲除，感染效率為80%，敲除率為72.2%。抑制RAD54B在A549細胞中的表達可顯著降低細胞增殖和caspase 3/7活性，并顯著提高細胞凋亡率。

結論:RAD54B在肺癌細胞增殖中具有致瘤作用。

該論文中，A549細胞的體外培養是使用Ausbian特級胎牛血清完成的。欲了解或購買Ausbian特級胎牛血清可以聯系北京締一生物400-166-8600.